Balances for Calibration

ISO 8655 recommends the selection of a suitable balance for pipette calibration based on the following criteria:

  • The standard uncertainty of measurement (or repeatability and linearity) shall not be more than two to three times resolution
  • The balance shall be equipped with an evaporation trap to minimise the loss of water by evaporation for small volumes 
  • An official traceable calibration certificate and calibration once a year is required 

Nominal Volume of a Pipette

Balance Resolution Repeatability and Linearity Standard Uncertainty of Measurement
1 μl ≤ V > 10 μl 0.001 mg 0.002 mg 0.002 mg
10 μl < V > 100 μl 0.01 mg 0.02 mg 0.02 mg
100 μl < V > 1000 μl 0.1 mg 0.2 mg 0.2 mg
1000 μl < V > 10 ml 0.1 mg 0.2 mg 0.2 mg
10 ml < V > 200 ml 1 mg 2 mg 2 mg