Calibration Levels Explained

Calibration levels are derived from a number of gravimetric weighings taken at different volumes. For variable volume pipettes it is recommended that weighings be taken at the nominal (maximum) volume, the mid-point (50% of nominal) and the minimum volume (10% of nominal). Weighings can also be taken at the maximum and minimum volumes only, or even at just the mid-point volume. For fixed volume pipettes, weighings can only be taken at the fixed volume.

For Best Results ISO 8655:2022 Recommends a 10@3 Calibration with PreCal
ISO 8655:2022 parts 2, 6 and 7 require tolerances for the nominal volume, 50% of nominal volume, and 10% of nominal volume. The tests must be done with at least 10 repetitions per tested volume and at three points, minimum.